Kinetic Chromogenic Formulation
Chromo-LAL lysate is lyophilized with substrate reagent and buffers. It is optimized for the kinetic chromogenic LAL test method in microplate readers. Chromo-LAL is a buffered, stable and robust lysate, suitable for quantitative testing of a wide range of samples.
Package Insert (PDF)
Kinetic Products Brochure (PDF)
The sensitivity for chromogenic assays is determined by the lowest standard concentration on the standard curve used for the assay. The maximum sensitivity of Chromo-LAL is 0.005 EU/mL.
Sample to Lysate Ratio
In the Chromo-LAL test, reconstituted Chromo-LAL reagent is used at a ratio of 1:1 and a volume of 100 µL.
The Chromo-LAL/sample mixture is incubated at 37 ± 1°C in a microplate reader equipped with a 405 nm filter. Software is used to construct the standard curve and calculate the endotoxin concentrations.
The reconstituted lysate is stable for 24 hours at 2–8°C or for two weeks at -20°C or colder if frozen immediately after reconstitution. Reconstituted Chromo-LAL lysate may be frozen and thawed once.
Chromo-LAL lysate is reconstituted with LAL Reagent Water (LRW). It can also be reconstituted with Glucashield®(1→3)-ß-D-glucan inhibiting buffer, to render the assay endotoxin specific.
Chromo-LAL lysate is sold by the vial for greater ordering and testing flexibility. This allows you to order the amount of lysate that is right for you.
3.2 mL/Vial 5 Pack (150 tests)