assay kit is specific for (1→3)-β-D-glucan. The assay is based upon a modification of the Limulus
Amebocyte Lysate (LAL) pathway. The Glucatell reagent is processed to eliminate Factor C, and is therefore specific for (1→3)-β-D-glucan. The reagent does not react to other polysaccharides, including beta-glucans with different glycosidic linkages.
Sample to Lysate RatioKinetic Assay:
25 µL of sample or standard is mixed with 100 µL of reagent in a microplate.
50 µL of sample is mixed with 50 µL of reagent in a microplate.
Test PerformanceKinetic Assay:
The Glucatell/sample mixture is incubated at 37°C ± 1°C in a microplate reader equipped with a 405 nm filter and analyzed over time by a suitable software program.
The Glucatell/sample mixture is placed in a
microplate heating block at 37°C ± 1°C for the recommended
time period. 50 µL each of the three diazo reagents are added
to the mixture to stop the reaction, which is then read at
540–550 nm. Concentration of the samples is analyzed by
a suitable software program.
Store all reagents at 2–8°C in the dark. Reconstituted Glucatell reagent should be stored at 2–8°C and used within 2 hours. Alternatively, reconstituted Glucatell reagent can be frozen at -20°C for 20 days, thawed once and used. The diazo-reagents should be used the day they are prepared.
The Glucatell kit is available as either an end-point or kinetic chromogenic assay for use in microplates. The kit contains the Glucatell reagent, a (1→3)-β-D-glucan standard, buffer, diazo reagents (end-point kit only), glucan-free water, and glucan-free microplates.
- Analyzing final products for (1→3)-β-D-glucan
- Investigating LAL Out of Specification results
- Qualifying raw materials
- Monitoring cellulosic filter extractables
- Monitoring fungal fermentation processes
- Analyzing fermentation and cell culture media
- Monitoring airborne glucan burden