Associates of Cape Cod, Inc.
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Testing Excellence
Product Description
Pyrochrome is a versatile quantitative chromogenic reagent that may be used to perform either kinetic or endpoint assays in microplate readers. The standard Pyrochrome test is read at 405 nm.Pyrochrome is also offered in a diazo kit for end-point tests. The diazo reagents shift the optical absorbance wavelength making it especially useful for testing samples with color interference. The test is read at 540–550 nm.
Product Sensitivity
The sensitivity for chromogenic assays is determined by the lowest standard concentration on the standard curve used for the assay. The maximum sensitivity of Pyrochrome is 0.005 EU/mL.Sample to Lysate Ratio
In the Pyrochrome test, reconstituted Pyrochrome LAL reagent can be used in 50 μL or 100 μL volumes in a sample:lysate ratio of 1:1.Test Performance
The Pyrochrome/sample mixture is incubated at 37± 1°C and read in a microplate reader equipped with a 405 nm filter or 540–550 nm filter for diazo method. Software is used to construct the standard curve and calculate the endotoxin concentrations.Product Stability
Pyrochrome must be used within 8 hours of reconstitution. Vials may be stored at 2–8ºC (or on ice). Reconstituted Pyrochrome cannot be frozen.Product Reconstitution
Pyrochrome lysate is reconstituted with an optimized reaction buffer, included with each vial of LAL Reagent. Pyrochrome can also be reconstituted with Glucashield® buffer, a (1→3)-ß-D-glucan inhibiting buffer, to render the assay endotoxin specific.Product Packaging
Pyrochrome® product line is offered with a matched buffer and is recommended for use with the 10 ng/vial Control Standard Endotoxin (EC010).Pyrochrome® is also offered in a diazo kit for end-point tests. The diazo reagents change the absorption maximum from 405 nm to 540—550 nm making it especially useful for testing samples with color interference.
Pyrochrome® | |
#C1500 | 60 Test |
#CD060 | 60 Test (with diazo-reagents) |